Mycobacteria protocols /

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Bibliographic Details
Imprint:	Totowa, N.J. : Humana Press, ©1998.
Description:	1 online resource (xiv, 472 pages) : illustrations
Language:	English
Series:	Methods in molecular biology ; v. 101 Methods in molecular biology (Clifton, N.J.) ; v. 101.
Subject:	Mycobacteria -- Laboratory manuals. Bacterial genetics -- Laboratory manuals. Mycobacterium -- genetics. DNA -- physiology. Molecular Biology -- methods. Mycobacterium -- growth & development. Bacterial genetics. Mycobacteria. Laboratory manuals.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/11128704

Hidden Bibliographic Details
Other authors / contributors:	Parish, Tanya. Stoker, Neil G.
ISBN:	0896034712 9780896034716 9781592595761 1592595766 128083689X 9781280836893 9786610836895 6610836892
Notes:	Includes bibliographical references and index. English. Print version record.
Summary:	In Mycobacteria Protocols, laboratory experts present cutting-edge molecular biology techniques for studying the mycobacteria, a medically significant group of organisms responsible for both human and animal disease-including tuberculosis and leprosy. The methods range from basic culture techniques, cell fractionation, and nucleic acid purification to more advanced protocols for the introduction of cloned DNA into mycobacteria, the use of reporter genes, and the expression of foreign genes. There are also important techniques for mycobacteriophages, genome analysis, chemical and transposon mutagenesis, gene replacement, and analysis of gene expression, as well as such clinically useful techniques as antibiotic susceptibility testing, speciation, and RFLP analysis. Mycobacteria Protocols contains a unique collection of both established and recently developed techniques that have been optimized and adapted for use with mycobacteria. Offering an eminently practical introduction for all those carrying out basic or applied research in this important class of bacteria, Mycobacteria Protocols is certain to become the new standard reference in the field, one from which even expert researchers will learn many time-saving techniques.
Other form:	Mycobacteria protocols
Standard no.:	10.1385/0896034712.

Table of Contents:

Mycobacteria : bugs and bugbears / Tanya Parish, Neil G. Stoker
Mycobacteria : general culture methodology and safety considerations / Bryan W. Allen
Isolation of genomic DNA from mycobacteria / John T. Belisle, Michael G. Sonnenberg
Nucleic-acid extraction : plasmid/cosmid DNA / Bonnie B. Plikaytis, C. Harold King
Pulsed field gel electrophoresis for mycobacteria / Wolfgang J. Philipp, Stephen Gordon, Amalio Telenti, Stewart T. Cole
Extraction of RNA from mycobacteria / Eshwar Mahenthiralingam
Preparation of cell-free extracts from mycobacteria / Tanya Parish, Paul R. Wheeler
Preparation of cell-wall fractions from mycobacteria / Gurdyal S. Besra
Using MycDB on the World-Wide Web / Staffan Bergh, Stewart T. Cole
Conjugating DNA into mycobacteria / Julie-Ann Gavigan, Carlos Martín
Electroporation of mycobacteria / Tanya Parish, Neil G. Stoker
Mycobacteriophages / Gary J. Sarkis, Graham F. Hatfull
Chemical mutagenesis of mycobacteria / Lucy A. Brooks
Tn611 transposon mutagenesis in Mycobacterium smegmatis using a temperature-sensitive delivery system / Esther Pérez, Julie-Ann Gavigan, Isabel Otal, Christophe Guilhot, Vladimir Pelicic, Brigitte Giquel, Carlos Martin
Homologous recombination in Mycobacterium smegmatis : screening methods for detection of gene replacement / Robert N. Husson
Gene replacement in Mycobacterium smegmatis using a dominant negative selectable marker / Peter Sander, Erik C. Böttger
Gene replacement in Mycobacterium intracellulare / Britt-Inger Marklund, Richard W. Stokes
Gene replacement in Mycobacterium bovis BCG / Elizabeth Norman
The application of bacterial luciferase as a reporter gene in mycobacteria / Stephen Gordon, Gavin Chung, Peter Andrew
Green fluorescent protein : a tool for gene expression and cell biology in mycobacteria / Laura E. Via, Subramanian Dhandayuthapani, Dusanka Deretic, V. Deretic
Expression of genes in mycobacteria / Peadar Ó Gaora
Secretion of mammalian proteins from mycobacteria / Peter J. Murray, Richard A. Young
Intracellular gene expression : analysis of RNA from mycobacteria in macrophages using RT-PCR / Philip D. Butcher, Joseph A. Mangan, Irene M. Monahan
Analysis of mycobacterial differential gene expression by RAP-PCR / Joseph A. Mangan, Philip D. Butcher
Mycobacterial speciation / Francis A. Drobniewski, Anne H. Uttley
Species identification of mycobacteria using rDNA sequencing / Philip Kirschner, Erik C. Böttger
Detection of Mycobacterium tuberculosis by a calorimetric polymerase chain reaction / Stuart M. Wilson
Rapid detection and simultaneous strain differentiation of Mycobacterium tuberculosis complex bacteria by spoligotyping / Henri O. Molhuizen, Annelies E. Bunschoten, Leo M. Schouls, Jan D. Embden
Computer analysis of IS6110 RFLP patterns of Mycobacterium tuberculosis / Herre F. Heersma, Kristin Kremer, Jan D. Embden
Detection of mutations in mycobacteria by PCR-SSCP (single-strand conformation polymorphism) / Amalio Telenti, Nadine Honoré, Stewart T. Cole
The use of luciferase-reporter phage for antibiotic-susceptibility testing of mycobacteria / Paul F. Riska, William R. Jacobs.

Mycobacteria protocols /

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