Oligonucleotide synthesis : methods and applications /

Oligonucleotide synthesis : methods and applications /

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Bibliographic Details
Imprint:Totowa, N.J. : Humana Press, ©2005.
Description:1 online resource (xvi, 435 pages) : illustrations
Language:English
Series:Methods in molecular biology ; 288
Methods in molecular biology (Clifton, N.J.) ; v. 288.
Subject:
Oligonucleotides -- Synthesis -- Laboratory manuals.
Oligonucleotides -- chemical synthesis
Oligonucleotides -- chemistry
Oligonucleotides -- Synthesis
Laboratory Manual
Laboratory manuals
Laboratory manuals.
Format: E-Resource Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/11137458
Hidden Bibliographic Details
Other authors / contributors:Herdewijn, Piet.
ISBN:1588292339
9781588292339
1592598234
9781592598236
1280359854
9781280359859
9786610359851
6610359857
Notes:Includes bibliographical references and index.
English.
Print version record.
Summary:Nucleic acids chemistry has progressed rapidly in the last 10 years with the introduction of many new techniques and the development of many novel therapeutic entities. In Oligonucleotide Synthesis: Methods and Applications, laboratory experts describe in step-by-step detail the powerful new techniques they have developed for oligonucleotide synthesis and for the use of modified oligonucleotides in biotechnology. Among the protocol highlights are the synthesis of phosphorothioates via new sulfur transfer agents, the synthesis of LNA, peptide conjugation methods to improve cellular delivery and cell-specific targeting, triple helix formation, and a novel two-step process that yields a high purity DNA that is less costly. Novel applications include the use of molecular beacons to monitor the PCR amplification process, nuclease footprinting to study the sequence-selective binding of small molecules to DNA, nucleic acid libraries, optimization of RNA synthesis for siRNA applications, and the use of small interference RNA (siRNA) as an inhibitor of gene expression. The protocols follow the successful Methods in Molecular Biology"!series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and highly practical, Oligonucleotide Synthesis: Methods and Applications offers today's investigators not only insight into important new biotechnologies, but also a full range of the detailed protocols needed to work successfully in DNA synthesis and its applications today
Other form:Print version: Oligonucleotide synthesis. Totowa, N.J. : Humana Press, ©2005 1588292339 1592598234
Standard no.:10.1385/1592598234.

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