Mast cells : methods and protocols /

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Bibliographic Details
Imprint:Totowa, N.J. : Humana Press, ©2006.
Description:1 online resource (xvii, 439 pages) : illustrations
Language:English
Series:Methods in molecular biology ; 315
Methods in molecular biology (Clifton, N.J.) ; v. 315.
Subject:
Format: E-Resource Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/11142519
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Other authors / contributors:Krishnaswamy, Guha.
Chi, David S.
ISBN:1588293742
9781588293749
1592599672
9781592599677
Notes:Includes bibliographical references and index.
Print version record.
Summary:The mast cell, long implicated in causing allergic reactions, may also be involved in many other disease processes, including cancer, heart disease, parasitic disease, atherosclerosis, asthma, and arthritis. In Mast Cells: Methods and Protocols, hands-on experts describe in detail their best techniques for the isolation, culture, and study of both activation and signaling in human mast cells. These readily reproducible methods take advantage of the latest advances in molecular biology, technology, and information science. The techniques provide a sound base of methodology for mast cell research and include methods for the identification of mast cells, the development of mast cells in vitro, the study of mast cell signaling and gene expression, and the measurement of mast cell expression of inflammatory mediators. Additional chapters cover methods for studying mast cell interactions with other cell types (endothelial cells, fibroblasts, and B cells), the roles of mast cells in host defense, and mast cell apoptosis. A survey of mast cell biology offers insight into its history and the implications for adaptive immunity. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and highly practical, Mast Cells: Methods and Protocols provides mast cell researchers with reproducible accounts of basic and advanced molecular and cellular techniques used in studying this fascinating, multifunctional cell.
Other form:Print version: Mast cells. Totowa, N.J. : Humana Press, ©2006 1588293742