Germ cell protocols. Part 1, Sperm and oocyte analysis /

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Bibliographic Details
Imprint:Totowa, N.J. : Humana Press, ©2004.
Description:1 online resource (1 volume) : illustrations (some color)
Language:English
Series:Methods in molecular biology ; v. 253, pt. 1
Methods in molecular biology (Clifton, N.J.) ; volume 253, pt. 1.
Subject:
Format: E-Resource Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/11154600
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Varying Form of Title:Sperm and oocyte analysis
Other authors / contributors:Schatten, Heide.
ISBN:1592597440
9781592597444
1588291219
Notes:Includes bibliographical references and indexes.
Print version record.
Summary:A gold-standard collection of readily reproducible techniques for the molecular and genetic analysis of germ cells in a variety of different reproductive systems. Volume 1: Sperm and Oocyte Analysis focuses on sperm cells, oocyte analysis, oocyte maturation, fertilization, and preparation techniques. Highlights include in vitro maturation and fertilization of human, porcine, and canine oocytes; the cryopreservation of sperm cells; establishment of an in vitro spermatogenesis system; visualization of sperm accessory structures; and motility assays of stallion spermatozoa. Each readily reproducible protocol is described in step-by-step detail and contains an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Other form:Print version: Germ cell protocols. Volume 1, Sperm and oocyte analysis. Totowa, N.J. : Humana Press, ©2004 1588291219 9781588291219