Germ cell protocols. Part 1, Sperm and oocyte analysis /
Saved in:
| Imprint: | Totowa, N.J. : Humana Press, ©2004. |
|---|---|
| Description: | 1 online resource (1 volume) : illustrations (some color) |
| Language: | English |
| Series: | Methods in molecular biology ; v. 253, pt. 1 Methods in molecular biology (Clifton, N.J.) ; volume 253, pt. 1. |
| Subject: | |
| Format: | E-Resource Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/11154600 |
MARC
| LEADER | 00000cam a2200000Ia 4500 | ||
|---|---|---|---|
| 001 | 11154600 | ||
| 005 | 20210625185342.9 | ||
| 006 | m o d | ||
| 007 | cr cnu---unuuu | ||
| 008 | 070911m20049999njua ob 001 0 eng d | ||
| 019 | |a 320966075 |a 648274708 |a 741252602 |a 756436698 | ||
| 020 | |a 1592597440 |q (electronic bk.) | ||
| 020 | |a 9781592597444 |q (electronic bk.) | ||
| 020 | |z 1588291219 |q (v. 1 ; |q alk. paper) | ||
| 035 | |a (OCoLC)170926184 |z (OCoLC)320966075 |z (OCoLC)648274708 |z (OCoLC)741252602 |z (OCoLC)756436698 | ||
| 035 | 9 | |a (OCLCCM-CC)170926184 | |
| 040 | |a N$T |b eng |e pn |c N$T |d YDXCP |d OCLCQ |d GW5XE |d IDEBK |d OCLCQ |d CUS |d OCLCQ |d CNTRU |d E7B |d OCLCO |d OCLCQ |d OCLCA |d OCLCQ |d VVL |d OCLCQ |d OCLCO |d OCLCQ |d OTZ |d STF |d OCLCA |d MERER |d OCLCQ |d U3W |d OCLCQ |d OCLCO |d GW5XE |d WURST |d OCLCA |d OCLCQ |d OCLCO | ||
| 049 | |a MAIN | ||
| 050 | 4 | |a SF767.5 | |
| 060 | 4 | |a W1 |b ME9616J v.253-254 | |
| 060 | 4 | |a WQ 25 |b G372 2004 | |
| 072 | 7 | |a MED |x 089020 |2 bisacsh | |
| 245 | 0 | 0 | |a Germ cell protocols. |p Part 1, |p Sperm and oocyte analysis / |c edited by Heide Schatten. |
| 246 | 3 | 0 | |a Sperm and oocyte analysis |
| 260 | |a Totowa, N.J. : |b Humana Press, |c ©2004. | ||
| 300 | |a 1 online resource (1 volume) : |b illustrations (some color) | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a computer |b c |2 rdamedia | ||
| 338 | |a online resource |b cr |2 rdacarrier | ||
| 490 | 1 | |a Methods in molecular biology ; |v v. 253, pt. 1 | |
| 504 | |a Includes bibliographical references and indexes. | ||
| 505 | 0 | |a Cryopreservation of semen of the salmonidae with special reference to large-scale fertilization / F. Lahnsteiner -- Fertilization and sperm chemotaxis in ascidians / M. Yoshida -- Assays for vertebrate sperm chemotaxis / H. Sugiyama [and others] -- Stallion spermatozoa viability: Comparison of flow cytometry with other methods / K. Merkies and M.M. Buhr -- Visualization of sperm accessory structures in the mammalian spermatids, spermatozoa, and zygotes by immunofluorescence, confocal, and immunoelectron microscopy / P. Sutovsky -- Localization of zona pellucida receptors on live sperm by fluorophore-conjugated solubilized zona pellucida proteins / H.R. Burkin, A. Paula Alves-Vieira and D.J. Miller -- Detection of human papillomavirus DNA in sperm using polymerase chain reaction / O.A. Olatunbosun, A.M. Case and H.G. Deneer -- Spermatogonial stem cells / K. Nayernia, M. Li and W. Engel -- Molecular and cellular approaches for establishment of an in vitro spermatogenesis system / K. Nayernia and W. Engel -- Acrosin activity assay for the evaluation of mammalian sperm acrosome reaction / Y. Lax, S. Rubinstein and H. Breitbart -- Isolation of sea urchin sperm plasma membranes / K.J. Mengerink and V.D. Vacquier -- Detection of centrosome structure in fertilized and artificially activated sea urchin eggs using immunofluorescence microscopy and isolation of centrosomes followed by structural characterization with field emission scanning electron microscopy / H. Schatten and A. Chakrabarti -- Microscopic techniques for studying sperm-oocyte interaction during fertilization and early embryonic development / M.W. Tengowski -- Immunocytochemical staining of the metaphase ii spindle in mammalian oocytes / S.F. Mullen and J.K. Critser -- Spindle imaging in living mammalian oocytes using the liquid-crystal polarized light microscope and its practical use / W.H. Wang, J. Gill and C. Boutin -- In vitro maturation and fertilization of pig oocytes / H.Y. Fan and Q.Y. Sun -- In vitro maturation and in vitro fertilization of human oocytes / W.H. Wang and J. Gill -- In vitro maturation and fertilization of canine oocytes / K. Reynaud, M. Saint-Dizier and S. Chastant-Maillard -- Detection and measurement of membrane-bound protein tyrosine kinases in the zebrafish egg / W. Wu and W.H. Kinsey -- Single-cell method for estimation of protein tyrosine kinases in the zebrafish egg / E. MacGregor and W.H. Kinsey -- Activity of MAPK/p90rsk during fertilization in mice, rats, and pigs / Q.Y. Sun and H.Y. Fan. | |
| 588 | 0 | |a Print version record. | |
| 520 | 3 | |a A gold-standard collection of readily reproducible techniques for the molecular and genetic analysis of germ cells in a variety of different reproductive systems. Volume 1: Sperm and Oocyte Analysis focuses on sperm cells, oocyte analysis, oocyte maturation, fertilization, and preparation techniques. Highlights include in vitro maturation and fertilization of human, porcine, and canine oocytes; the cryopreservation of sperm cells; establishment of an in vitro spermatogenesis system; visualization of sperm accessory structures; and motility assays of stallion spermatozoa. Each readily reproducible protocol is described in step-by-step detail and contains an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. | |
| 650 | 0 | |a Veterinary embryology |x Research |x Technique. | |
| 650 | 0 | |a Germ cells |x Research |x Technique. | |
| 650 | 1 | 2 | |a Cytological Techniques. |
| 650 | 1 | 2 | |a Germ Cells. |
| 650 | 2 | 2 | |a Genetic Techniques. |
| 650 | 7 | |a MEDICAL |x Veterinary Medicine |x Food Animal. |2 bisacsh | |
| 653 | 0 | 0 | |a kiemcellen |
| 653 | 0 | 0 | |a germ cells |
| 653 | 0 | 0 | |a spermatozoa |
| 653 | 0 | 0 | |a oocytes |
| 653 | 0 | 0 | |a laboratoriummethoden |
| 653 | 0 | 0 | |a laboratory methods |
| 653 | 0 | 0 | |a technieken |
| 653 | 0 | 0 | |a techniques |
| 653 | 0 | 0 | |a rijpen |
| 653 | 0 | 0 | |a maturation |
| 653 | 0 | 0 | |a in vitro bevruchting |
| 653 | 0 | 0 | |a in vitro fertilization |
| 653 | 0 | 0 | |a genetische analyse |
| 653 | 0 | 0 | |a genetic analysis |
| 653 | 0 | 0 | |a celbiologie |
| 653 | 0 | 0 | |a cellular biology |
| 653 | 0 | 0 | |a moleculaire biologie |
| 653 | 0 | 0 | |a molecular biology |
| 653 | 0 | 0 | |a protocollen |
| 653 | 0 | 0 | |a protocols |
| 653 | 1 | 0 | |a Reproduction and Fertility |
| 653 | 2 | 0 | |a Cellular Biology |
| 653 | 1 | 0 | |a Voortplanting en vruchtbaarheid |
| 653 | 2 | 0 | |a Celbiologie |
| 655 | 4 | |a Electronic books. | |
| 700 | 1 | |a Schatten, Heide. |0 http://id.loc.gov/authorities/names/n88044841 | |
| 776 | 0 | 8 | |i Print version: |t Germ cell protocols. Volume 1, Sperm and oocyte analysis. |d Totowa, N.J. : Humana Press, ©2004 |z 1588291219 |z 9781588291219 |w (DLC) 2003023511 |w (OCoLC)53325045 |
| 830 | 0 | |a Methods in molecular biology (Clifton, N.J.) ; |v volume 253, pt. 1. | |
| 903 | |a HeVa | ||
| 929 | |a oclccm | ||
| 999 | f | f | |i 0130a76c-dc1e-5c88-ac0c-e270d51db281 |s 7d0e45af-86ac-5703-88ab-e454a4587a04 |
| 928 | |t Library of Congress classification |a SF767.5 |l Online |c UC-FullText |u https://link.springer.com/10.1385/1592597440 |z Springer Nature |g ebooks |i 12530854 | ||