Germ cell protocols. Part 1, Sperm and oocyte analysis /
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Imprint: | Totowa, N.J. : Humana Press, ©2004. |
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Description: | 1 online resource (1 volume) : illustrations (some color) |
Language: | English |
Series: | Methods in molecular biology ; v. 253, pt. 1 Methods in molecular biology (Clifton, N.J.) ; volume 253, pt. 1. |
Subject: | |
Format: | E-Resource Book |
URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/11154600 |
Varying Form of Title: | Sperm and oocyte analysis |
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Other authors / contributors: | Schatten, Heide. |
ISBN: | 1592597440 9781592597444 1588291219 |
Notes: | Includes bibliographical references and indexes. Print version record. |
Summary: | A gold-standard collection of readily reproducible techniques for the molecular and genetic analysis of germ cells in a variety of different reproductive systems. Volume 1: Sperm and Oocyte Analysis focuses on sperm cells, oocyte analysis, oocyte maturation, fertilization, and preparation techniques. Highlights include in vitro maturation and fertilization of human, porcine, and canine oocytes; the cryopreservation of sperm cells; establishment of an in vitro spermatogenesis system; visualization of sperm accessory structures; and motility assays of stallion spermatozoa. Each readily reproducible protocol is described in step-by-step detail and contains an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. |
Other form: | Print version: Germ cell protocols. Volume 1, Sperm and oocyte analysis. Totowa, N.J. : Humana Press, ©2004 1588291219 9781588291219 |
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