Directed enzyme evolution : screening and selection methods /

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Bibliographic Details
Imprint:Totowa, N.J. : Humana Press, ©2003.
Description:1 online resource (xv, 383 pages) : illustrations (some color)
Language:English
Series:Methods in molecular biology ; 230
Methods in molecular biology (Clifton, N.J.) ; v. 230.
Subject:
Format: E-Resource Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/11154991
Hidden Bibliographic Details
Other authors / contributors:Arnold, Frances Hamilton.
Georgiou, George.
ISBN:1592593968
9781592593965
1280842709
9781280842702
9786610842704
6610842701
158829286X
9781588292865
Notes:Includes bibliographical references and index.
English.
Print version record.
Summary:Directed evolution, the application of evolutionary design to enzyme engineering, requires effective screening strategies to isolate those proteins that perform a desired function from the libraries generated by the techniques. In Directed Enzyme Evolution: Screening and Selection Methods, seasoned practitioners from many leading laboratories describe their leading and readily reproducible screening strategies for isolating useful clones. These techniques have been optimized for sensitivity, high throughput, and robustness, and are of proven utility for directed evolution purposes. The assays presented use a variety of techniques, including genetic complementation, microtiter plates, solid-phase screens with colorimetric substrates, and flow cytometric screens. There are also representative examples of how phage libraries may be interrogated for enzymatic activity. Each protocol contains detailed step-by-step instructions and many notes on how best to deal with the problems that may occur. An accompanying volume, Directed Evolution Library Creation: Methods and Protocols (ISBN 1-58829-285-1), describes readily reproducible methods for the creation of mutated DNA molecules and DNA libraries. Taken together, Directed Enzyme Evolution: Screening and Selection Methods and Directed Evolution Library Creation: Methods and Protocols capture for newcomers and more experienced investigators alike all the key methods for using directed protein evolution to better understand protein structure-function relationships, to discover new enzymes and therapeutic proteins, and to design new assays suitable for specific applications.
Other form:Print version: Directed enzyme evolution. Totowa, N.J. : Humana Press, ©2003 158829286X 9781588292865
Standard no.:10.1385/1592593968.