Transcription factor protocols /

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Bibliographic Details
Imprint:Totowa, N.J. : Humana Press, ©2000.
Description:1 online resource (x, 306 pages) : illustrations
Language:English
Series:Methods in molecular biology ; v. 130
Methods in molecular biology (Clifton, N.J.) ; v. 130.
Subject:
Format: E-Resource Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/11155061
Hidden Bibliographic Details
Other authors / contributors:Tymms, Martin J.
ISBN:9781592596867
159259686X
1280832878
9781280832871
9786610832873
6610832870
9781617370816
0896035735
9780896035737
Notes:Includes bibliographical references and index.
Electronic reproduction. [Place of publication not identified] : HathiTrust Digital Library, 2010.
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212
English.
digitized 2010 HathiTrust Digital Library committed to preserve
Print version record.
Summary:In Transcription Factor Protocols, Martin Tymms has created a powerful compendium of the major techniques for the study of those DNA sequences and protein factors that regulate the transcription of protein encoding genes. With chapters contributed by leading investigators well versed in the methods, this eminently practical compendium includes not only well established protocols, but also novel techniques that are now being widely adopted. Among the important new methods treated are the use of triplex-forming oligonucleotides, the application of whole genome PCR to the isolation of gene promoters/enhancers, the analysis of in vivo methylation, and in vivo footprinting using UV light and ligation-mediated PCR. Step-by-step instructions, tips about pitfalls to avoid, and extensive procedural notes all help to ensure robust and reproducible results. Transcription Factor Protocols offers both experienced workers and new researchers a vital first-stop reference collection of the core techniques for all those exploring the role of transcription factors in gene regulation today.
Other form:Print version: Transcription factor protocols. Totowa, N.J. : Humana Press, ©2000 0896035735 9780896035737
Standard no.:10.1385/159259686X

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