Nuclear transfer protocols : cell reprogramming and transgenesis /

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Bibliographic Details
Imprint:Totowa, N.J. : Humana Press, ©2006.
Description:1 online resource (xi, 337 pages) : illustrations
Language:English
Series:Methods in molecular biology, 1064-3745 ; 348
Methods in molecular biology (Clifton, N.J.) ; v. 348.
Subject:
Format: E-Resource Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/11155219
Hidden Bibliographic Details
Other authors / contributors:Verma, Paul J.
Trounson, Alan.
ISBN:1588292800
1597451541
9781597451543
9781588292803
1280832444
9781280832444
9786610832446
6610832447
Digital file characteristics:text file
PDF
Notes:Includes bibliographical references and index.
English.
Print version record.
Summary:Nuclear Transfer Protocols: Cell Reprogramming and Transgenesis is a comprehensive review of nuclear transfer technology in vertebrates, aimed at reprogramming differentiated nuclei and effecting targeted gene transfer. The authors provide readily reproducible techniques for the generation of cloned embryos and animals in a number of key research and commercially important vertebrates. Additional chapters provide alternative cutting-edge methods for nuclear transfer, such as zona-free nuclear transfer. Of immense benefit are descriptions of procedures associated with cloning, such as in vitro maturation of oocytes, activation and culture of cloned embryos, maintenance of pregnancy, and neonatal care of clones.
Other form:Print version: Nuclear transfer protocols. Totowa, N.J. : Humana Press, ©2006 1588292800 9781588292803
Standard no.:10.1007/978-1-59745-154-3.