Transmembrane signaling protocols /

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Bibliographic Details
Edition:2nd ed.
Imprint:Totowa, N.J. : Humana Press, ©2006.
Description:1 online resource (xi, 364 pages) : illustrations
Language:English
Series:Methods in molecular biology, 1064-3745 ; 332
Methods in molecular biology (Clifton, N.J.) ; v. 332.
Subject:
Format: E-Resource Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/11179320
Hidden Bibliographic Details
Other authors / contributors:Ali, Hydar.
Haribabu, Bodduluri.
ISBN:158829546X
9781588295460
9781597450485
1597450480
9786610832033
661083203X
1280832037
9781280832031
Digital file characteristics:text file PDF
Notes:Includes bibliographical references and index.
English.
Print version record.
Summary:The complete sequencing of the human genome and many new microarray and proteomic methods for analyzing global changes in both gene expression and protein profiles have greatly increased our ability to understand transmembrane signaling processes in close detail. In this fully updated and expanded second edition of Transmembrane Signaling Protocols, leading experts demonstrate the use of such techniques as single-cell imaging, bioluminescence resonance energy transfer, and global proteomics in the study of transmembrane signaling events. Highlights include the functional expression and genetic selection of mutant mammalian transmembrane receptors in yeast, real-time analysis of transmembrane receptor signaling in live cells, and bioluminescence energy transfer to monitor protein-protein interaction. Additional chapters use state-of-the-art methods to analyze gene expression and proteomic profiles related to transmembrane signaling events, as well as to genetically reconstitute bone marrow for the study of signal transduction ex vivo. Introductory chapters provide a wide-ranging perspective, including an overview of the many recent advances in our understanding of signal transduction pathways. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and highly practical, Transmembrane Signaling Protocols, Second Edition, makes available to novice and experienced researchers alike the readily reproducible techniques needed to advance the study of signal transduction pathways today.
Other form:Print version: Transmembrane signaling protocols. 2nd ed. Totowa, N.J. : Humana Press, ©2006 158829546X
Standard no.:10.1385/1597450480

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