The inflammasome : methods and protocols /

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Bibliographic Details
Imprint:New York : Humana Press : Springer, [2013]
©2013
Description:1 online resource (xi, 225 pages) : illustrations
Language:English
Series:Methods in molecular biology, 1940-6029 ; volume no. 1040
Methods in molecular biology (Clifton, N.J.) ; v. 1040.
Subject:
Format: E-Resource Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/11200455
Hidden Bibliographic Details
Other authors / contributors:De Nardo, Christine M., editor.
Latz, Eicke, editor.
ISBN:9781627035231
1627035230
9781627035224
1627035222
Digital file characteristics:PDF
text file
Notes:Includes bibliographical references and index.
English.
Print version record.
Summary:Inflammasomes have a seemingly simple architecture.
A sensor molecule of the NLR or PYHIN protein family recruits the common adapter molecule apoptosis-associated speck-like protein containing a CARD (ASC).
Even so, inflammasome activation is highly regulated at multiple steps and precise details of how inflammasomes are activated at the molecular level remain largely unknown.
In The Inflammasome: Methods and Protocols, expert researchers in the field detail methods and protocols to study multiple aspects of inflammasome function.
These methods include the generation of inflammasome stimuli, perturbance of cellular homeostasis, assessment of ASC speck formation, monitoring of caspase-1 activity and processing, activation of IL-1beta cytokines, and analysis of inflammasome assembly.
Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Inflammasomes have a seemingly simple architecture. A sensor molecule of the NLR or PYHIN protein family recruits the common adapter molecule apoptosis-associated speck-like protein containing a CARD (ASC). Even so, inflammasome activation is highly regulated at multiple steps and precise details of how inflammasomes are activated at the molecular level remain largely unknown. In The Inflammasome: Methods and Protocols, expert researchers in the field detail methods and protocols to study multiple aspects of inflammasome function. These methods include the generation of inflammasome stimuli, perturbance of cellular homeostasis, assessment of ASC speck formation, monitoring of caspase-1 activity and processing, activation of IL-1β cytokines, and analysis of inflammasome assembly. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Other form:Print version: Inflammasome. New York : Humana Press ; Springer, ©2013 9781627035224
Standard no.:10.1007/978-1-62703-523-1