DNA cloning and assembly methods /

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Bibliographic Details
Imprint:New York : Humana Press, [2014]
©2014
Description:1 online resource (xi, 303 pages) : illustrations (some color)
Language:English
Series:Methods in Molecular Biology, 1940-6029 ; 1116
Methods in molecular biology (Clifton, N.J.) ; v. 1116.
Subject:
Format: E-Resource Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/11225311
Hidden Bibliographic Details
Other authors / contributors:Valla, Svein, editor.
Lale, Rahmi, editor.
ISBN:9781627037648
1627037640
1627037632
9781627037631
9781627037631
Digital file characteristics:text file PDF
Notes:Includes bibliographical references and index.
English.
Online resource; title from PDF title page (SpringerLink, viewed January 13, 2014).
Summary:In DNA Cloning and Assembly Methods, expert researchers in the field detail many of the methods which are now commonly used for DNA cloning and make cloning procedures faster, more reliable and also suitable for high-throughput handling. These include methods and protocols that are based on several mechanisms including type II and IIS restriction enzymes, single stranded annealing, sequence overlap, and recombination. With additional chapters on software programs that are suitable for primer design, a feature crucial for the functionality of the described methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, DNA Cloning and Assembly Methods seeks to provide scientist with a valuable and useful resource for wet lab researchers within life sciences.
Other form:Print version: DNA cloning and assembly methods. New York : Humana Press, [2014] 1627037632
Standard no.:10.1007/978-1-62703-764-8