Plant epigenetics : methods and protocols /

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Bibliographic Details
Imprint:New York : Humana Press, ©2010.
Description:1 online resource (x, 273 pages) : illustrations
Language:English
Series:Springer protocols
Methods in molecular biology, 1064-3745 ; v. 631
Springer protocols (Series)
Methods in molecular biology (Clifton, N.J.) ; v. 631.
Subject:
Format: E-Resource Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/11228148
Hidden Bibliographic Details
Other authors / contributors:Kovalchuk, Igor.
Zemp, Franz J.
ISBN:9781607616467
1607616467
9781607616450
1607616459
Digital file characteristics:text file PDF
Notes:Includes bibliographical references and index.
English.
Print version record.
Summary:The past fifteen years have witnessed major advances in epigenetics, one of the most popular and quickly evolving fields of modern science. In Plant Epigenetics: Methods and Protocols, expert researchers explore the most recent developments, examining in great detail the contribution of epigenetic regulation to cell function in plants. Chapters include a variety of protocols for studying the function of small non-coding RNAs, DNA methylation, and histone modifications in plants, often in different degrees of complexity. This volume describes bioinformatic approaches to the analysis of high-throughput data, such as bisulfite sequencing and Chip-on-chip assays. It features much-desired protocols for plant transgenesis and the analysis of genome stability, with a detailed discussion of their applications to epigenetic studies. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Wide-ranging and innovative, Plant Epigenetics: Methods and Protocols is an invaluable manual designed to help researchers uncover the undiscovered and unexplained phenomena in plant biology.
Other form:Print version: Plant epigenetics. New York : Humana Press, ©2010 9781607616450 1607616459
Standard no.:10.1007/978-1-60761-646-7

MARC

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245 0 0 |a Plant epigenetics :  |b methods and protocols /  |c edited by Igor Kovalchuk, Franz J. Zemp. 
260 |a New York :  |b Humana Press,  |c ©2010. 
300 |a 1 online resource (x, 273 pages) :  |b illustrations 
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490 1 |a Methods in molecular biology,  |x 1064-3745 ;  |v v. 631 
504 |a Includes bibliographical references and index. 
505 0 |a Analysis of DNA methylation in plants by bisulfite sequencing / Andrea Foerster and Ortrun Mittelsten Scheid -- Analysis of bisulfite sequencing data from plant DNA using CyMATE / Andrea M. Foerster [and others] -- Analysis of locus-specific changes in methylation patterns using a COBRA (combined bisulfite restriction analysis) assay / Alex Boyko and Igor Kovalchuk -- Detection of changes in global genome methylation using the cytosine-extension assay / Alex Boyko and Igor Kovalchuk -- In situ analysis of DNA methylation in plants / Palak Kathiria and Igor Kovalchuk -- Analysis of mutation/rearrangement frequencies and methylation patterns at a given DNA locus using restriction fragment length polymorphism / Alex Boyko and Igor Kovalchuk -- Isoschizomers and amplified fragment length polymorphism for the detection of specific cytosine methylation changes / Leonor Ruiz-García [and others] -- Analysis of small RNA populations using hybridization to DNA tiling arrays / Martine Boccara [and others] -- Northern blotting techniques for small RNAs / Todd Blevins -- qRT-PCR of small RNAs / Erika Varkonyi-Gasic and Roger P. Hellens -- Cloning new small RNA sequences / Yuko Tagami, Naoko Inaba, and Yuichiro Watanabe -- Genome-wide mapping of protein-DNA interaction by chromatin immunoprecipitatino and DNA microarray hybridization (ChIP-chip). Part A : ChiP-chip molecular methods / Julia J. Reimer and Franziska Turck. Part B : ChIP-chip data analysis / Ulrike Göbel, Julia Reimer, and Franziska Turck -- Metaanalysis of ChIP-chip data / Julia Engelhorn and Franziska Turck -- Chromatin immunoprecipitation protocol for histone modifications and protein-DNA binding analyses in Arabidopsis / Stéphane Pien and Ueli Grossniklaus -- cDNA libraries for virus-induced gene silencing / Andrea T. Todd, Enwu Liu, and Jonathan E. Page -- Detection and quantification of DNA strand breaks using the ROPS (random oligonucleotide primed synthesis) assay / Alex Boyko and Igor Kovalchuk -- Reporter gene-based recombination lines for studies of genome stability / Palak Kathiria and Igor Kovalchuk -- Plant transgenesis / Alicja Ziemienowicz. 
588 0 |a Print version record. 
520 |a The past fifteen years have witnessed major advances in epigenetics, one of the most popular and quickly evolving fields of modern science. In Plant Epigenetics: Methods and Protocols, expert researchers explore the most recent developments, examining in great detail the contribution of epigenetic regulation to cell function in plants. Chapters include a variety of protocols for studying the function of small non-coding RNAs, DNA methylation, and histone modifications in plants, often in different degrees of complexity. This volume describes bioinformatic approaches to the analysis of high-throughput data, such as bisulfite sequencing and Chip-on-chip assays. It features much-desired protocols for plant transgenesis and the analysis of genome stability, with a detailed discussion of their applications to epigenetic studies. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Wide-ranging and innovative, Plant Epigenetics: Methods and Protocols is an invaluable manual designed to help researchers uncover the undiscovered and unexplained phenomena in plant biology. 
546 |a English. 
650 0 |a Plant genetic regulation.  |0 http://id.loc.gov/authorities/subjects/sh90005025 
650 0 |a Epigenesis.  |0 http://id.loc.gov/authorities/subjects/sh85044382 
650 2 |a Epigenesis, Genetic. 
650 2 |a Gene Expression Regulation, Plant. 
650 2 |a Gene Silencing. 
650 2 |a Gene Expression Regulation. 
650 2 |a Genetic Phenomena. 
650 2 |a Phenomena and Processes 
650 7 |a Epigenesis.  |2 fast  |0 (OCoLC)fst00914128 
650 7 |a Plant genetic regulation.  |2 fast  |0 (OCoLC)fst01065456 
653 0 0 |a planten 
653 0 0 |a plants 
653 0 0 |a epigenetica 
653 0 0 |a epigenetics 
653 0 0 |a moleculaire genetica 
653 0 0 |a molecular genetics 
653 0 0 |a dna methylation 
653 0 0 |a bioinformatics 
653 0 0 |a histonen 
653 0 0 |a histones 
653 0 0 |a dna sequencing 
653 0 0 |a transgene planten 
653 0 0 |a transgenic plants 
653 0 0 |a laboratoriummethoden 
653 0 0 |a laboratory methods 
653 0 0 |a protocollen 
653 0 0 |a protocols 
653 1 0 |a Biological Techniques 
653 1 0 |a Biologische technieken 
655 4 |a Electronic books. 
700 1 |a Kovalchuk, Igor.  |0 http://id.loc.gov/authorities/names/n2009184182 
700 1 |a Zemp, Franz J. 
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