Plant epigenetics : methods and protocols /
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Imprint: | New York : Humana Press, ©2010. |
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Description: | 1 online resource (x, 273 pages) : illustrations |
Language: | English |
Series: | Springer protocols Methods in molecular biology, 1064-3745 ; v. 631 Springer protocols (Series) Methods in molecular biology (Clifton, N.J.) ; v. 631. |
Subject: | |
Format: | E-Resource Book |
URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/11228148 |
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245 | 0 | 0 | |a Plant epigenetics : |b methods and protocols / |c edited by Igor Kovalchuk, Franz J. Zemp. |
260 | |a New York : |b Humana Press, |c ©2010. | ||
300 | |a 1 online resource (x, 273 pages) : |b illustrations | ||
336 | |a text |b txt |2 rdacontent | ||
337 | |a computer |b c |2 rdamedia | ||
338 | |a online resource |b cr |2 rdacarrier | ||
347 | |a text file |b PDF |2 rda | ||
490 | 1 | |a Springer protocols | |
490 | 1 | |a Methods in molecular biology, |x 1064-3745 ; |v v. 631 | |
504 | |a Includes bibliographical references and index. | ||
505 | 0 | |a Analysis of DNA methylation in plants by bisulfite sequencing / Andrea Foerster and Ortrun Mittelsten Scheid -- Analysis of bisulfite sequencing data from plant DNA using CyMATE / Andrea M. Foerster [and others] -- Analysis of locus-specific changes in methylation patterns using a COBRA (combined bisulfite restriction analysis) assay / Alex Boyko and Igor Kovalchuk -- Detection of changes in global genome methylation using the cytosine-extension assay / Alex Boyko and Igor Kovalchuk -- In situ analysis of DNA methylation in plants / Palak Kathiria and Igor Kovalchuk -- Analysis of mutation/rearrangement frequencies and methylation patterns at a given DNA locus using restriction fragment length polymorphism / Alex Boyko and Igor Kovalchuk -- Isoschizomers and amplified fragment length polymorphism for the detection of specific cytosine methylation changes / Leonor Ruiz-García [and others] -- Analysis of small RNA populations using hybridization to DNA tiling arrays / Martine Boccara [and others] -- Northern blotting techniques for small RNAs / Todd Blevins -- qRT-PCR of small RNAs / Erika Varkonyi-Gasic and Roger P. Hellens -- Cloning new small RNA sequences / Yuko Tagami, Naoko Inaba, and Yuichiro Watanabe -- Genome-wide mapping of protein-DNA interaction by chromatin immunoprecipitatino and DNA microarray hybridization (ChIP-chip). Part A : ChiP-chip molecular methods / Julia J. Reimer and Franziska Turck. Part B : ChIP-chip data analysis / Ulrike Göbel, Julia Reimer, and Franziska Turck -- Metaanalysis of ChIP-chip data / Julia Engelhorn and Franziska Turck -- Chromatin immunoprecipitation protocol for histone modifications and protein-DNA binding analyses in Arabidopsis / Stéphane Pien and Ueli Grossniklaus -- cDNA libraries for virus-induced gene silencing / Andrea T. Todd, Enwu Liu, and Jonathan E. Page -- Detection and quantification of DNA strand breaks using the ROPS (random oligonucleotide primed synthesis) assay / Alex Boyko and Igor Kovalchuk -- Reporter gene-based recombination lines for studies of genome stability / Palak Kathiria and Igor Kovalchuk -- Plant transgenesis / Alicja Ziemienowicz. | |
588 | 0 | |a Print version record. | |
520 | |a The past fifteen years have witnessed major advances in epigenetics, one of the most popular and quickly evolving fields of modern science. In Plant Epigenetics: Methods and Protocols, expert researchers explore the most recent developments, examining in great detail the contribution of epigenetic regulation to cell function in plants. Chapters include a variety of protocols for studying the function of small non-coding RNAs, DNA methylation, and histone modifications in plants, often in different degrees of complexity. This volume describes bioinformatic approaches to the analysis of high-throughput data, such as bisulfite sequencing and Chip-on-chip assays. It features much-desired protocols for plant transgenesis and the analysis of genome stability, with a detailed discussion of their applications to epigenetic studies. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Wide-ranging and innovative, Plant Epigenetics: Methods and Protocols is an invaluable manual designed to help researchers uncover the undiscovered and unexplained phenomena in plant biology. | ||
546 | |a English. | ||
650 | 0 | |a Plant genetic regulation. |0 http://id.loc.gov/authorities/subjects/sh90005025 | |
650 | 0 | |a Epigenesis. |0 http://id.loc.gov/authorities/subjects/sh85044382 | |
650 | 2 | |a Epigenesis, Genetic. | |
650 | 2 | |a Gene Expression Regulation, Plant. | |
650 | 2 | |a Gene Silencing. | |
650 | 2 | |a Gene Expression Regulation. | |
650 | 2 | |a Genetic Phenomena. | |
650 | 2 | |a Phenomena and Processes | |
650 | 7 | |a Epigenesis. |2 fast |0 (OCoLC)fst00914128 | |
650 | 7 | |a Plant genetic regulation. |2 fast |0 (OCoLC)fst01065456 | |
653 | 0 | 0 | |a planten |
653 | 0 | 0 | |a plants |
653 | 0 | 0 | |a epigenetica |
653 | 0 | 0 | |a epigenetics |
653 | 0 | 0 | |a moleculaire genetica |
653 | 0 | 0 | |a molecular genetics |
653 | 0 | 0 | |a dna methylation |
653 | 0 | 0 | |a bioinformatics |
653 | 0 | 0 | |a histonen |
653 | 0 | 0 | |a histones |
653 | 0 | 0 | |a dna sequencing |
653 | 0 | 0 | |a transgene planten |
653 | 0 | 0 | |a transgenic plants |
653 | 0 | 0 | |a laboratoriummethoden |
653 | 0 | 0 | |a laboratory methods |
653 | 0 | 0 | |a protocollen |
653 | 0 | 0 | |a protocols |
653 | 1 | 0 | |a Biological Techniques |
653 | 1 | 0 | |a Biologische technieken |
655 | 4 | |a Electronic books. | |
700 | 1 | |a Kovalchuk, Igor. |0 http://id.loc.gov/authorities/names/n2009184182 | |
700 | 1 | |a Zemp, Franz J. | |
776 | 0 | 8 | |i Print version: |t Plant epigenetics. |d New York : Humana Press, ©2010 |z 9781607616450 |z 1607616459 |w (DLC) 2010922364 |w (OCoLC)471801564 |
830 | 0 | |a Springer protocols (Series) | |
830 | 0 | |a Methods in molecular biology (Clifton, N.J.) ; |v v. 631. | |
903 | |a HeVa | ||
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928 | |t Library of Congress classification |a QK981.4 .P53 2010 |l Online |c UC-FullText |u https://link.springer.com/10.1007/978-1-60761-646-7 |z Springer Nature |g ebooks |i 12531398 |