Heterologous gene expression in E. coli : methods and protocols /

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Bibliographic Details
Imprint:New York, NY : Humana Press, ©2011.
Description:1 online resource (xi, 310 pages) : illustrations (some color)
Language:English
Series:Springer protocols
Methods in molecular biology, 1064-3745 ; 705
Springer protocols (Series)
Methods in molecular biology (Clifton, N.J.) ; v. 705.
Subject:
Format: E-Resource Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/11244952
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Other authors / contributors:Evans, Thomas C.
Xu, Ming-Qun.
ISBN:9781617379673
1617379670
9781617379666
1617379662
Digital file characteristics:text file PDF
Notes:Includes bibliographical references and index.
English.
Print version record.
Summary:Protein expression in a heterologous host is a cornerstone of biomedical research and of the biotechnology industry. Despite the advanced state of protein expression technology improvements are still needed. For example, membrane proteins constitute a significant percentage of the total cellular proteins but as a class are very difficult to overexpress, especially in a heterologous host. The ideal host would have the ability to express any protein, with relevant post-translational modifications, and be as easy to work with as E. coli. In Heterologous Gene Expression in E. coli: Methods and Protocols, expert scientists intimately familiar with the relevant techniques offer chapters that greatly expand the utility of this expression host. The contributions in this detailed volume describe methods, for example, to successfully express proteins in E. coli that would otherwise form aggregates in this host, to add post-translational modifications, to incorporate non-standard amino acid residues or moieties into E. coli expressed proteins, to identify binding partners, and to express membrane proteins. Written in the highly successful Methods in Molecular Biology format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and cutting-edge, Heterologous Gene Expression in E. coli: Methods and Protocols seeks to familiarize the researcher with the myriad of E. coli expression strains available and move E. coli closer to that ideal of the perfect host.
Other form:Print version: Heterologous gene expression in E. coli. New York, NY : Humana Press, ©2011
Standard no.:10.1007/978-1-61737-967-3