Bacterial protein secretion systems : methods and protocols /

Bacterial protein secretion systems : methods and protocols /

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Bibliographic Details
Imprint:New York, NY : Humana Press : Springer, ©2017.
Description:1 online resource (xv, 528 pages) : illustrations (some color)
Language:English
Series:Methods in molecular biology, 1064-3745 ; v. 1615
Methods in molecular biology (Clifton, N.J.) ; v. 1615.
Subject:
Bacterial proteins -- Laboratory manuals.
Bacteriological Techniques.
Bacterial Proteins -- metabolism.
Clinical Laboratory Techniques.
Bacterial proteins.
Laboratory Manual.
Laboratory manuals.
Format: E-Resource Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/11308564
Hidden Bibliographic Details
Other authors / contributors:Journet, Laure, editor.
Cascales, Eric, editor.
ISBN:9781493970339
149397033X
9781493970315
1493970313
Digital file characteristics:text file PDF
Notes:Includes bibliographical references and index.
Online resource; title from PDF title page (SpringerProtocol, viewed July 5, 2017).
Summary:This volume details protocols that cover the broad arsenal of techniques used to study a secretion system from A to Z. Chapters focus on identifying and localizing the different subunits, defining interactions within subunits, monitoring conformational changes, purifying and imaging of large complexes, defining the assembly pathway by fluorescence microscopy and the role of energy during assembly and/or secretion, identifying secreted effectors as well as reporters to follow effector transport. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Bacterial Protein Secretion Systems: Methods and Protocol aims to provide techniques that are not restricted to the study of secretion systems but are also of specific interest for any researcher interested on multi-protein complexes of the bacterial cell envelope.
Other form:Print 9781493970315
Standard no.:10.1007/978-1-4939-7033-9

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