Flow cytometry basics for the non-expert /

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Bibliographic Details
Author / Creator:Goetz, Christine, author.
Imprint:Cham : Springer, 2018.
Description:1 online resource (229 pages)
Language:English
Series:Techniques in Life Science and Biomedicine for the Non-Expert
Techniques in life science and biomedicine for the non-expert.
Subject:
Format: E-Resource Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/11737611
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Other authors / contributors:Hammerbeck, Christopher.
Bonnevier, Jody.
ISBN:9783319980713
3319980718
9783319980706
331998070X
Digital file characteristics:text file PDF
Notes:1 Antibody Development for Flow Cytometry.
Print version record.
Summary:Flow cytometry allows for the measurement of different aspects of a cell or particles in real time. Applications include quantifying various types of T cells to determine the presence of various immune disorders, such as whether HIV has progressed to AIDS, studying the dynamics of immune signaling in cells to detect the suppression of normal immune function, and analyzing biopsy specimens for tumors, to aid in cancer diagnosis and prognosis. In addition to simplifying the technique and using vocabulary accessible to those not trained in cell biology and immunology, the book includes the following 4 unqiue features: 1) Tips on how to set up an experiment for flow cytometry optimially 2) An expanded data set of flow staining including multiparameter flow cytometry 3) Detailed staining protocols for flow cytometry (ex. protocols optimized for transcription factors, secreted cytokines, phospho-antibodies, and surface antigens) 4) Section devoted to antibody development and conjugation.
Other form:Print version: Goetz, Christine. Flow Cytometry Basics for the Non-Expert. Cham : Springer, ©2018 9783319980706
Standard no.:10.1007/978-3-319-98071-3