Flow cytometry basics for the non-expert /
Saved in:
| Author / Creator: | Goetz, Christine, author. |
|---|---|
| Imprint: | Cham : Springer, 2018. |
| Description: | 1 online resource (229 pages) |
| Language: | English |
| Series: | Techniques in Life Science and Biomedicine for the Non-Expert Techniques in life science and biomedicine for the non-expert. |
| Subject: | |
| Format: | E-Resource Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/11737611 |
MARC
| LEADER | 00000cam a2200000Mi 4500 | ||
|---|---|---|---|
| 001 | 11737611 | ||
| 005 | 20210625185237.1 | ||
| 006 | m o d | ||
| 007 | cr un|---aucuu | ||
| 008 | 181124s2018 sz o 000 0 eng d | ||
| 015 | |a GBB8O1894 |2 bnb | ||
| 016 | 7 | |a 019138886 |2 Uk | |
| 019 | |a 1075608988 |a 1079793948 |a 1080606426 |a 1086474138 |a 1110215537 | ||
| 020 | |a 9783319980713 |q (electronic bk.) | ||
| 020 | |a 3319980718 |q (electronic bk.) | ||
| 020 | |z 9783319980706 | ||
| 020 | |z 331998070X | ||
| 024 | 7 | |a 10.1007/978-3-319-98071-3 |2 doi | |
| 035 | |a (OCoLC)1076245284 |z (OCoLC)1075608988 |z (OCoLC)1079793948 |z (OCoLC)1080606426 |z (OCoLC)1086474138 |z (OCoLC)1110215537 | ||
| 035 | 9 | |a (OCLCCM-CC)1076245284 | |
| 037 | |a com.springer.onix.9783319980713 |b Springer Nature | ||
| 040 | |a EBLCP |b eng |e rda |e pn |c EBLCP |d GW5XE |d N$T |d OCLCQ |d YDX |d NOC |d DKU |d UPM |d UAB |d OCLCF |d OH1 |d UKMGB |d CAUOI |d VT2 |d UKAHL |d LEAUB |d OCLCQ |d UX1 | ||
| 049 | |a MAIN | ||
| 050 | 4 | |a QH585.5.F56 |b G64 2018 | |
| 060 | 0 | 0 | |a 2019 I-243 |
| 060 | 1 | 0 | |a QY 95 |
| 072 | 7 | |a HEA |x 039000 |2 bisacsh | |
| 072 | 7 | |a MED |x 014000 |2 bisacsh | |
| 072 | 7 | |a MED |x 022000 |2 bisacsh | |
| 072 | 7 | |a MED |x 112000 |2 bisacsh | |
| 072 | 7 | |a MED |x 045000 |2 bisacsh | |
| 072 | 7 | |a MJCM |2 bicssc | |
| 072 | 7 | |a MJCM |2 thema | |
| 100 | 1 | |a Goetz, Christine, |e author. | |
| 245 | 1 | 0 | |a Flow cytometry basics for the non-expert / |c Christine Goetz, Christopher Hammerbeck, Jody Bonnevier. |
| 264 | 1 | |a Cham : |b Springer, |c 2018. | |
| 300 | |a 1 online resource (229 pages) | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a computer |b c |2 rdamedia | ||
| 338 | |a online resource |b cr |2 rdacarrier | ||
| 347 | |a text file |b PDF |2 rda | ||
| 490 | 1 | |a Techniques in Life Science and Biomedicine for the Non-Expert | |
| 588 | 0 | |a Print version record. | |
| 505 | 0 | |a Intro; Preface; Acknowledgments; Contents; About the Book; Chapter 1: Flow Cytometry: Definition, History, and Uses in Biological Research; 1 Components of Flow Cytometry; 2 Definition of Flow Cytometry; 3 History of Flow Cytometry; 3.1 Cellular Impedance and the Coulter Principle; 3.2 Fluorescence-Based Flow Cytometers; 3.3 Monoclonal and Polyclonal Antibodies; 3.4 Fluorescent Molecules; 3.5 Fluorescence Activated Cell Sorting (FACS); 4 Modern Flow Cytometers; 4.1 Fluidic-Based Flow Cytometers; 4.2 Acoustic-Based Flow Cytometers; 4.3 Mass Cytometers (CyTOF); References. | |
| 505 | 8 | |a Chapter 2: Physics of a Flow Cytometer1 Components of Fluidic-Based Flow Cytometers; 1.1 Fluidics; 1.2 Optics; 1.3 Electronics; 2 Putting It All Together; 3 Materials; 3.1 Flow Antibodies; 3.2 Cell Activation Reagents; 3.3 Cell Purification Kits; 3.4 Staining Buffers/Fc Block Reagents; References; Chapter 3: The Language of Flow Cytometry and Experimental Setup; 1 Flow Data Plots and Gating; 1.1 Flow Data Plots; 1.2 Gating; 2 Mean Fluorescence Intensity (MFI) and Biexponential vs. Logarithmic Scaling; 2.1 Mean Fluorescence Intensity (MFI); 2.2 Logarithmic vs. Biexponential Scaling. | |
| 505 | 8 | |a 3 Forward Scatter (FSC)/Side Scatter (SSC) and Doublet Exclusion3.1 FSC/SSC; 3.2 Doublet Exclusion; 4 CS & T and Basic Experimental Setup; 4.1 CS 4.2 Basic Experimental Setup; 4.2.1 Experimental/Flow Panel Design; 4.2.2 Surface and Intracellular Staining on iTregs; 4.2.3 Experimental Setup on the Fortessa; 4.2.4 Sample Acquisition and Analysis; 5 Basic Concepts of Compensation; 6 Materials; 6.1 Flow Antibodies; 6.2 Cell Activation Reagents; 6.3 Cell Purification Kits; 6.4 Staining Buffers/Fc Block Reagents; References; Chapter 4: Fluorochrome Choices for Flow Cytometry. | |
| 505 | 8 | |a 1 Fluorochromes and Their Pros and Cons2 Spectrum Viewers and How to Use Them; 3 Fluorochrome Combinations to Use/Avoid Together; 4 Laser Strength on the Fortessa vs. the Calibur; 5 High- vs. Low-Density Cell Markers and Fluorochrome Choice; 6 Materials; 6.1 Flow Antibodies; 6.2 Staining Buffers/Fc Block Reagents; References; Chapter 5: Using the Process of Compensation to Prevent False Positive Data Caused by Fluorescence Spillover: A Practical Example; 1 Compensation: A Practical Example; 2 Manual Compensation by Comparing the Median Fluorescence Intensity of Cell Populations. | |
| 505 | 8 | |a 3 Considerations When Performing Compensation3.1 Using the Same Compensation Settings for Multiple Experiments; 3.2 Dimming of Fluorochromes as a Result of Compensation; 3.3 Consider the Number of Overlapping Fluorochromes; 3.4 Consider If Compensation Is Necessary; 3.5 Consider If Fluorochromes with High Spectral Overlap Can Be Separated on Two Different Cell Types; 3.6 Using Cells as Single-Stain Controls vs. Beads; 4 Materials; 4.1 Flow Antibodies; 4.2 Staining Buffers/Fc Block Reagents; References; Chapter 6: Primary and Secondary Antibodies and Flow Cytometry Controls. | |
| 500 | |a 1 Antibody Development for Flow Cytometry. | ||
| 520 | |a Flow cytometry allows for the measurement of different aspects of a cell or particles in real time. Applications include quantifying various types of T cells to determine the presence of various immune disorders, such as whether HIV has progressed to AIDS, studying the dynamics of immune signaling in cells to detect the suppression of normal immune function, and analyzing biopsy specimens for tumors, to aid in cancer diagnosis and prognosis. In addition to simplifying the technique and using vocabulary accessible to those not trained in cell biology and immunology, the book includes the following 4 unqiue features: 1) Tips on how to set up an experiment for flow cytometry optimially 2) An expanded data set of flow staining including multiparameter flow cytometry 3) Detailed staining protocols for flow cytometry (ex. protocols optimized for transcription factors, secreted cytokines, phospho-antibodies, and surface antigens) 4) Section devoted to antibody development and conjugation. | ||
| 650 | 0 | |a Flow cytometry. |0 http://id.loc.gov/authorities/subjects/sh85049287 | |
| 650 | 7 | |a Medical laboratory testing & techniques. |2 bicssc | |
| 650 | 7 | |a Immunology. |2 bicssc | |
| 650 | 7 | |a HEALTH & FITNESS |x Diseases |x General. |2 bisacsh | |
| 650 | 7 | |a MEDICAL |x Clinical Medicine. |2 bisacsh | |
| 650 | 7 | |a MEDICAL |x Diseases. |2 bisacsh | |
| 650 | 7 | |a MEDICAL |x Evidence-Based Medicine. |2 bisacsh | |
| 650 | 7 | |a MEDICAL |x Internal Medicine. |2 bisacsh | |
| 650 | 7 | |a Flow cytometry. |2 fast |0 (OCoLC)fst00927770 | |
| 655 | 4 | |a Electronic books. | |
| 700 | 1 | |a Hammerbeck, Christopher. | |
| 700 | 1 | |a Bonnevier, Jody. | |
| 776 | 0 | 8 | |i Print version: |a Goetz, Christine. |t Flow Cytometry Basics for the Non-Expert. |d Cham : Springer, ©2018 |z 9783319980706 |
| 830 | 0 | |a Techniques in life science and biomedicine for the non-expert. |0 http://id.loc.gov/authorities/names/n2016187336 | |
| 903 | |a HeVa | ||
| 929 | |a oclccm | ||
| 999 | f | f | |i 2f57b7a0-7bad-5d97-b291-731a557a3973 |s 44480042-9e66-5e7d-8fb2-c4f7611b7f6c |
| 928 | |t Library of Congress classification |a QH585.5.F56 G64 2018 |l Online |c UC-FullText |u https://link.springer.com/10.1007/978-3-319-98071-3 |z Springer Nature |g ebooks |i 12558430 | ||