Microbial metabolic engineering : methods and protocols /

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Bibliographic Details
Imprint:New York, N.Y. : Humana Press : Springer, [2019]
©2019
Description:1 online resource (x, 252 pages) : illustrations (some color)
Language:English
Series:Methods in Molecular Biology, 1940-6029 ; v. 1927
Methods in molecular biology (Clifton, N.J.) ; v. 1927.
Subject:
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/11792650
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Other authors / contributors:Santos, Christine Nicole S., editor.
Ajikumar, Parayil Kumaran, editor.
ISBN:9781493991426
1493991426
9781493991419
1493991418
Digital file characteristics:text file PDF
Notes:Includes bibliographical references and index.
Online resource; title from PDF title page (SpringerLink, viewed February 21, 2019).
Summary:This volume covers a wide array of topics that will aid researchers in the task of engineering complex biological systems. This book is divided into three parts: Part One discusses the discovery and identification of relevant biosynthetic pathways for engineering; Part Two looks at the development of genetic tools for manipulating enzymes, biosynthetic pathways, and whole genomes; and Part Three covers the characterization of engineered microbes using targeted and global systems biology tools, as well as in silico models. Chapters explore topics such as leveraging enzyme promiscuity to construct novel biosynthetic pathways; assembling combinatorial multigene pathways for rapid strain optimization; applying 'omics technologies for identifying bottlenecks; and engineering nontraditional host organisms like cyanobacterium and Yarrowia lipolytica. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting edge and authoritative, Microbial Metabolic Engineering: Methods and Protocols is a valuable resource for researchers and scientists interested in engineering and optimizing microbes for a variety of biotechnological applications.
Other form:Print version: Microbial metabolic engineering. New York, N.Y : Springer, ©2019 9781493991419
Standard no.:10.1007/978-1-4939-9142-6