In situ-downstream processing of recombinant histidine-tagged proteins from cultivations of Bacillus megaterium.

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Bibliographic Details
Author / Creator:	Gädke, Johannes.
Imprint:	Göttingen : Cuvillier Verlag, 2017.
Description:	1 online resource (129 pages)
Language:	English
Series:	Schriftenreihe des Institutes für Bioverfahrenstechnik der Technischen Universität Braunschweig ; v. 79 Schriftenreihe des Institutes für Bioverfahrenstechnik der Technischen Universität Braunschweig.
Subject:	Bacillus (Bacteria) Bacillus megaterium. Bacillus (Bacteria) Bacillus megaterium.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/12350835

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ISBN:	3736985517 9783736985513 9783736995512
Notes:	Includes bibliographical references. Print version record.
Summary:	Purification of (recombinant) proteins for industrial and pharmaceutical use is accompanied by high costs and difficulties in the scale-up with the necessity of many purification steps. This thesis demonstrates the use of functionalized superparamagnetic iron oxide nanoparticles for the purification of recombinant histidine-tagged proteins directly from a growing culture of the gram-positive bacterium Bacillus megaterium. The separation was performed using commercial hand held magnets. Regenerability and reusability are shown in shake flask scale. Automation of the process is demonstrated at lab scale bioreactors using two model proteins, Protein A and the antibody fragment α-Lysozyme D1.3scFv. The process demonstrates a quick and easy way to yield a product of high purity within a short period of time.
Other form:	Print version: Gädke, Johannes. In situ-downstream processing of recombinant histidine-tagged proteins from cultivations of Bacillus megaterium. Göttingen : Cuvillier Verlag, ©2017 9783736995512

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