DNA modification detection methods /

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Bibliographic Details
Imprint:New York, NY : Humana Press, [2022]
Description:1 online resource (vi, 194 pages) : illustrations (some color).
Language:English
Series:Springer protocols handbooks, 1949-2448
Springer protocols (Series),
Subject:
Format: E-Resource Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/12616799
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Other authors / contributors:Yuan, Bi-Feng, editor.
ISBN:9781071612293
1071612298
9781071612286
Notes:Online resource; title from PDF title page (SpringerLink, viewed July 22, 2021).
Summary:Understanding the functional roles of DNA modifications relies on the accurate detection, quantification, and mapping of DNA modifications. Methods for deciphering DNA modifications have substantially improved over the last several years, which greatly revolutionize the field of DNA modifications. In addition to DNA cytosine methylation (5-methylcytosine, 5mC), the best-characterized epigenetic modification, many new modifications have been discovered to present in DNA in recent years. This book provides a comprehensive overview of available techniques and methods together with detailed step-by-step protocols for experimental procedures required to successfully perform analysis on various types of DNA modifications, including 5mC, 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), 5-carboxycytosine (5caC), 5-hydroxymethyluracil (5hmU), 5-formyluracil (5fU), N6-methyladenine (6mA), [beta]-D-glucosyl-5-hydroxymethyluracil (base J) and 8-oxo-7,8-dihydroguanine (OG). This laboratory manual is a valuable source for biochemists and molecular biologists from different fields who wish to investigate DNA modifications.
Standard no.:10.1007/978-1-0716-1229-3