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| LEADER |
00000cam a22000004a 4500 |
| 001 |
7368689 |
| 003 |
ICU |
| 005 |
20090421191500.0 |
| 008 |
080717s2009 nyua b 001 0 eng |
| 010 |
|
|
|a 2008031690
|
| 020 |
|
|
|a 9780879698683 (hardcover : alk. paper)
|
| 020 |
|
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|a 0879698683 (hardcover : alk. paper)
|
| 020 |
|
|
|a 9780879698676 (pbk. : alk. paper)
|
| 020 |
|
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|a 0879698675 (pbk. : alk. paper)
|
| 035 |
|
|
|a 2008031690
|
| 035 |
|
|
|a (OCoLC)235945971
|
| 040 |
|
|
|a DNLM/DLC
|c DLC
|d NLM
|d YDXCP
|d BTCTA
|d C#P
|d UtOrBLW
|
| 042 |
|
|
|a pcc
|
| 050 |
0 |
0 |
|a QP551
|b .B265 2009
|
| 060 |
1 |
0 |
|a QU 25
|b B3115 2009
|
| 082 |
0 |
0 |
|a 572/.6078
|2 22
|
| 245 |
0 |
0 |
|a Basic methods in protein purification and analysis :
|b a laboratory manual /
|c edited by Richard J. Simpson, Peter D. Adams, and Erica A. Golemis.
|
| 260 |
|
|
|a Cold Spring Harbor, N.Y. :
|b Cold Spring Harbor Laboratory Press,
|c c2009.
|
| 300 |
|
|
|a vii, 436 p. :
|b ill. ;
|c 28 cm.
|
| 336 |
|
|
|a text
|b txt
|2 rdacontent
|0 http://id.loc.gov/vocabulary/contentTypes/txt
|
| 337 |
|
|
|a unmediated
|b n
|2 rdamedia
|0 http://id.loc.gov/vocabulary/mediaTypes/n
|
| 338 |
|
|
|a volume
|b nc
|2 rdacarrier
|0 http://id.loc.gov/vocabulary/carriers/nc
|
| 504 |
|
|
|a Includes bibliographical references and index.
|
| 505 |
0 |
|
|a Introduction to protein purification strategies -- Preparation of cellular and subcellular extracts -- One-dimensional polyacrylamide gel electrophoresis -- Preparative two-dimensional gel electrophoresis with immobilized pH gradients -- Ion-exchange chromatography -- Size-exclusion chromatography -- Reversed-phase high-performance liquid chromatography -- Affinity and immunoaffinity chromatography -- Metal chelate affinity chromatography -- Multidimensional chromatography of intact proteins -- Identification of protein-protein interactions by conventional column chromatography -- Identification of associated proteins by coimmunoprecipitation -- Chromatin immunoprecipitation of protein complexes -- Identification of protein-protein interactions with glutathione-s-transferase fusion proteins -- Direct visualization of protein interactions in living cells using bimolecular fluorescence complementation analysis -- In silico tools : analysis for creating focused interaction networks.
|
| 650 |
|
0 |
|a Proteins
|x Purification
|v Laboratory manuals.
|
| 650 |
|
0 |
|a Proteins
|x Analysis
|v Laboratory manuals.
|
| 650 |
1 |
2 |
|a Proteins
|x isolation & purification
|v laboratory manuals.
|
| 650 |
2 |
2 |
|a Chromatography
|x methods
|v Laboratory Manuals.
|
| 650 |
2 |
2 |
|a Electrophoresis
|x methods
|v Laboratory Manuals.
|
| 650 |
2 |
2 |
|a Immunoprecipitation
|x methods
|v Laboratory Manuals.
|
| 650 |
2 |
2 |
|a Proteins
|x analysis
|v Laboratory Manuals.
|
| 650 |
|
7 |
|a Proteins
|x Analysis.
|2 fast
|0 http://id.worldcat.org/fast/fst01079714
|
| 650 |
|
7 |
|a Proteins
|x Purification.
|2 fast
|0 http://id.worldcat.org/fast/fst01079750
|
| 655 |
|
7 |
|a Laboratory manuals.
|2 fast
|0 http://id.worldcat.org/fast/fst01920776
|
| 700 |
1 |
|
|a Simpson, Richard J.
|0 http://id.loc.gov/authorities/names/n2002160131
|1 http://viaf.org/viaf/21510769
|
| 700 |
1 |
|
|a Adams, Peter D.
|q (Peter David)
|0 http://id.loc.gov/authorities/names/n2005032051
|1 http://viaf.org/viaf/15055800
|
| 700 |
1 |
|
|a Golemis, Erica.
|0 http://id.loc.gov/authorities/names/n2001014471
|1 http://viaf.org/viaf/74070814
|
| 903 |
|
|
|a HeVa
|
| 929 |
|
|
|a cat
|
| 999 |
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|i d7751fc1-63b3-53ee-8c88-b841412d1465
|s 8aba61d9-1b6e-59a8-bedf-c0c2f7dbdb27
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| 928 |
|
|
|t Library of Congress classification
|a QP551 .B265 2009
|l JCL
|c JCL-Sci
|i 5449013
|
| 927 |
|
|
|t Library of Congress classification
|a QP551 .B265 2009
|l JCL
|c JCL-Sci
|e DOED
|e CRERAR
|b 088427650
|i 8493053
|
