Base editors : methods and protocols /

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Bibliographic Details
Imprint:New York, NY : Humana Press, [2023]
©2023
Description:1 online resource (xii, 235 pages) : illustrations (some color).
Language:English
Series:Methods in molecular biology, 1940-6029 ; 2606
Methods in molecular biology (Clifton, N.J.) ; 2606.
Subject:
Format: E-Resource Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/13548281
Hidden Bibliographic Details
Other authors / contributors:Bae, Sangsu, editor.
Song, Beomjong, editor.
ISBN:9781071628799
1071628798
9781071628782
107162878X
Notes:Includes index.
Online resource; title from PDF title page (SpringerLink, viewed January 12, 2023).
Summary:This volume explores base editors (BEs), an invaluable CRISPR-based genome editing tool with a wide variety of versatile applications. Beginning with an overview of BEs, their diverse variants, and computational tools, the book continues with experimental applications of BEs for disease modeling in mammalian cells and generating mutagenic mice, therapeutic base editing strategies, which covers delivery methods of BE-encoded DNA plasmids, mRNAs, or ribonucleoproteins through viruses or non-viral lipid nanoparticles, and lastly, the use of BEs in plants and bacteria. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Base Editors: Methods and Protocols serves as an ideal guide for researchers looking to use base editors to continue their studies in an array of fields.
Other form:Original 107162878X 9781071628782
Standard no.:10.1007/978-1-0716-2879-9